Klemm EJ, Gkrania-Klotsas E, Hadfield J, Forbester JL, Harris SR, Hale C, Heath JN, Wileman T, Clare S, Kane L, Goulding D, Otto TD, Kay S, Doffinger R, Cooke FJ, Carmichael A, Lever AM, Parkhill J, MacLennan CA, Kumararatne D, Dougan G, Kingsley RA. Emergence of host-adapted Salmonella Enteritidis through rapid evolution in an immunocompromised host. Nat Microbiol. 2016 Mar;1(3). pii: 15023. Epub 2016 Jan 25
Thomas DC, Clare S, Sowerby JM, Pardo M, Juss JK, Goulding DA, van der Weyden L, Storisteanu D, Prakash A, Espéli M, Flint S, Lee JC, Hoenderdos K, Kane L, Harcourt K, Mukhopadhyay S, Umrania Y, Antrobus R, Nathan JA, Adams DJ, Bateman A, Choudhary JS, Lyons PA, Condliffe AM, Chilvers ER, Dougan G*, Smith KG*. Eros is a novel transmembrane protein that controls the phagocyte respiratory burst and is essential for innate immunity. J Exp Med. 2017 Apr 3;214(4):1111-1128.
Alasoo K, Rodrigues J, Mukhopadhyay S, Knights AJ, Mann AL, Kundu K; HIPSCI Consortium, Hale C, Dougan G, Gaffney DJ. Shared genetic effects on chromatin and gene expression indicate a role for enhancer priming in immune response. Nat Genet. 2018 50:424-431.
Klemm EJ, Shakoor S, Page AJ, Qamar FN, Judge K, Saeed DK, Wong VK, Dallman TJ, Nair S, Baker S, Shaheen G, Qureshi S, Yousafzai MT, Saleem MK, Hasan Z, Dougan G, Hasan R Emergence of an extensively drug-resistant Salmonella enterica serovar Typhi clone harboring a promiscuous plasmid encoding resistance to fluoroquinolones and third-generation cephalosporins. MBio. 2018 9. pii: e00105-18.
Forbester JL, Lees EA, Goulding D, Forrest S, Yeung A, Speak A, Clare S, Coomber EL, Mukhopadhyay S, Kraiczy J, Schreiber F, Lawley TD, Hancock REW, Uhlig HH, Zilbauer M, Powrie F, Dougan G. Interleukin-22 promotes phagolysosomal fusion to induce protection against Salmonella entericaTyphimurium in human epithelial cells. Proc Natl Acad Sci U S A. 2018 115:10118-10123.
Klemm EJ, Wong VK, Dougan G. Emergence of dominant multidrug-resistant bacterial clades: Lessons from history and whole-genome sequencing. Proc Natl Acad Sci U S A. 2018; 115:12872-12877. PMID: 30559200
Ellington MJ, Heinz E, Wailan AM, Dorman MJ, de Goffau M, Cain AK, Henson SP, Gleadall N, Boinett CJ, Dougan G, Brown NM, Woodford N, Parkhill J, Török ME, Peacock SJ, Thomson NR. Contrasting patterns of longitudinal population dynamics and antimicrobial resistance mechanisms in two priority bacterial pathogens over 7 years in a single center. Genome Biol. 2019 Sep 2;20(1):184. doi: 10.1186/s13059-019-1785-1.
Kumar N, Browne HP, Viciani E, Forster SC, Clare S, Harcourt K, Stares MD, Dougan G, Fairley DJ, Roberts P, Pirmohamed M, Clokie MRJ, Jensen MBF, Hargreaves KR, Ip M, Wieler LH, Seyboldt C, Norén T, Riley TV, Kuijper EJ, Wren BW, Lawley TD. Adaptation of host transmission cycle during Clostridium difficile speciation. Nat Genet. 2019 Sep;51(9):1315-1320. doi: 10.1038/s41588-019-0478-8. Epub 2019 Aug 12.
Gibani MM, Jones E, Barton A, Jin C, Meek J, Camara S, Galal U, Heinz E, Rosenberg-Hasson Y, Obermoser G, Jones C, Campbell D, Black C, Thomaides-Brears H, Darlow C, Dold C, Silva-Reyes L, Blackwell L, Lara-Tejero M, Jiao X, Stack G, Blohmke CJ, Hill J, Angus B, Dougan G, Galán J, Pollard AJ. Investigation of the role of typhoid toxin in acute typhoid fever in a human challenge model. Nat Med. 2019 Jul;25(7):1082-1088. doi: 10.1038/s41591-019-0505-4. Epub 2019 Jul 3
Davies MR, McIntyre L, Mutreja A, Lacey JA, Lees JA, Towers RJ, Duchêne S, Smeesters PR, Frost HR, Price DJ, Holden MTG, David S, Giffard PM, Worthing KA, Seale AC, Berkley JA, Harris SR, Rivera-Hernandez T, Berking O, Cork AJ, Torres RSLA, Lithgow T, Strugnell RA, Bergmann R, Nitsche-Schmitz P, Chhatwal GS, Bentley SD, Fraser JD, Moreland NJ, Carapetis JR, Steer AC, Parkhill J, Saul A, Williamson DA, Currie BJ, Tong SYC, Dougan G, Walker MJ. Atlas of group A streptococcal vaccine candidates compiled using large-scale comparative genomics. Nat Genet. 2019 Jun;51(6):1035-1043. doi: 10.1038/s41588-019-0417-8. Epub 2019 May 27
Lees EA, Forbester JL, Forrest S, Kane L, Goulding D, Dougan G. Epithelial Cell Protection Against Salmonella and Other Pathogens. J Vis Exp. 2019 May 12;(147). doi: 10.3791/59478
Dunstan RA, Pickard D, Dougan S, Goulding D, Cormie C, Hardy J, Li F, Grinter R, Harcourt K, Yu L, Song J, Schreiber F, Choudhary J, Clare S, Coulibaly F, Strugnell RA, Dougan G, Lithgow T. The flagellotropic bacteriophage YSD1 targets Salmonella Typhi with a Chi-like protein tail fibre. Mol Microbiol. 2019 Sep 25. doi: 10.1111/mmi.14396. [Epub ahead of print)
Yeung ATY, Choi YH, Lee AHY, Hale C, Ponstingl H, Pickard D, Goulding D, Thomas M, Gill E, Kim JK, Bradley A, Hancock REW, Dougan G. A Genome-Wide Knockout Screen in Human Macrophages Identified Host Factors Modulating Salmonella Infection. MBio. 2019 Oct 8;10(5). pii: e02169-19
Jeon HJ, Im J, Haselbeck A, Holm M, Rakotozandrindrainy R, Bassiahi AS, Panzner U, Mogeni OD, Seo HJ, Lunguya O, Jacobs J, Okeke IN, Terferi M, Owusu-Dabo E, Dougan G, Carey M, Steele AD, Kim JH, Clemens JD, Andrews JR, Park SE, Baker S, Marks F. How Can the Typhoid Fever Surveillance in Africa and the Severe Typhoid Fever in Africa Programs Contribute to the Introduction of Typhoid Conjugate Vaccines? Clin Infect Dis. 2019 Oct 30;69(Supplement_6):S417-S421
Mukhopadhyay S, Heinz E, Porreca I, Alasoo K, Yeung A, Yang HT, Schwerd T, Forbester JL, Hale C, Agu CA, Choi YH, Rodrigues J, Capitani M, Jostins-Dean L, Thomas DC, Travis S, Gaffney D, Skarnes WC, Thomson N, Uhlig HH, Dougan G, Powrie F. Loss of IL-10 signaling in macrophages limits bacterial killing driven by prostaglandin E2. J Exp Med. 2020 3;217
Cader MZ, de Almeida Rodrigues RP, West JA, Sewell GW, Md-Ibrahim MN, Reikine S, Sirago G, Unger LW, Iglesias-Romero AB, Ramshorn K, Haag LM, Saveljeva S, Ebel JF, Rosenstiel P, Kaneider NC, Lee JC, Lawley TD, Bradley A, Dougan G, Modis Y, Griffin JL, Kaser A. FAMIN Is a Multifunctional Purine Enzyme Enabling the Purine Nucleotide Cycle. Cell. 2020 23;180:278-295.e23
Pragasam AK, Pickard D, Wong V, Dougan G, Kang G, Thompson A, John J, Balaji V, Mutreja A. Phylogenetic Analysis Indicates a Longer Term Presence of the Globally Distributed H58 Haplotype of Salmonella Typhi in Southern India. Clin Infect Dis. 2020 Jan 31
Klemm EJ, Shakoor S, Page AJ, Naz Qamar F, Judge K, Saeed DK, Wong VK et al, Gordon Dougan*, Rumina Hasan*. Emergence of an Extensively Drug-Resistant Salmonella enterica Serovar Typhi Clone Harboring a Promiscuous Plasmid Encoding Resistance to Fluoroquinolones and Third-Generation Cephalosporins. mBio. 2018 Jan-Feb; 9(1): e00105-18. Published online 2018 Feb 20. doi: 10.1128/mBio.00105-18 PMCID: PMC5821095
Weill FX, Domman D, Njamkepo E, Tarr C, Rauzier J, Fawal N et al, Dougan G, Grimont PAD, Parkhill J, Holt KE, Piarroux R, Ramamurthy T, Quilici ML, Thomson NR. Genomic history of the seventh pandemic of cholera in Africa. Science. 2017 Nov 10;358(6364):785-789. PMID:29123067
Thomas DC, Clare S, Sowerby JM, Pardo M, Juss JK, Goulding DA, van der Weyden L, Storisteanu D, Prakash A, Espéli M et al Dougan G*, Smith KG*. Eros is a novel transmembrane protein that controls the phagocyte respiratory burst and is essential for innate immunity. J Exp Med. 2017 Apr 3;214(4):1111-1128. PMID:28351984
First description of a novel gene controlling the oxidative burst in mammals. Contains key EM images
Klemm EJ, Gkrania-Klotsas E, Hadfield J, Forbester JL, Harris SR, Hale C, Heath JN, Wileman T, et al Dougan G*, Kingsley RA*. Emergence of host-adapted Salmonella Enteritidis through rapid evolution in an immunocompromised host. Nat Microbiol. 2016 Mar;1(3). pii: 15023. Epub 2016 Jan 25 PMID:27127642
Joint last author on a paper defining rapid in vivo evolution in a patient with key images
Forbester JL, Goulding D, Vallier L, Hannan N, Hale C, Pickard D, Mukhopadhyay S, Dougan G.The interaction of Salmonella enterica Serovar Typhimurium with intestinal organoids derived from human induced pluripotent stem cells. Infect Immun. 2015 pii: I&I.00161-15. PMID: 25964470
Imaging of Salmonella in intestinal organoids
Wong VK, Baker S, Pickard DJ, Parkhill J, Page AJ, Feasey NA, Kingsley RA, Thomson NR, Keane JA, et al, Dougan G. Phylogeographical analysis of the dominant multidrug-resistant H58 clade of Salmonella Typhi identifies inter- and intracontinental transmission events. Nat Genet. 2015 May 11. doi: 10.1038/ng.3281 PMID: 25961941
Comprehensive description of an AMR clade of typhoid
Yeung AT, Hale C, Xia J, Tate PH, Goulding D, Keane JA, Mukhopadhyay S, Forrester L, Billker O, Skarnes WC, Hancock RE, Dougan G. Conditional-ready mouse embryonic stem cell derived macrophages enable the study of essential genes in macrophage function. Sci Rep. 2015;5:8908 PMID:25752829
Imaging of mouse ES derived macrophages
von Mentzer A, Connor TR, Wieler LH, Semmler T, Iguchi A, Thomson NR, Rasko DA, Joffre E, Corander J, Pickard D, Wiklund G, Svennerholm AM, Sjöling A, Dougan G. Identification of enterotoxigenic Escherichia coli (ETEC) clades with long-term global distribution. Nat Genet. 2014 doi:10.1038/ng.3145. PMID:25383970
Definition of the population structure of human enterotoxigenic E. coli
Okoro CK, Kingsley RA, Connor TR, Harris SR, Parry CM, Al-Mashhadani MN, Kariuki S, Msefula CL, Gordon MA et al & Dougan G. Intracontinental spread of human invasive Salmonella Typhimurium pathovariants in sub-Saharan Africa. Nat Genet. 2012 44:1215-21. PMID: 25961941
Definition of a new form of Salmonellosis in Sub-Saharan Africa
Lawley TD, Clare S, Walker AW, Stares MD, Connor TR, Raisen C, Goulding D, Rad R, Schreiber F, Brandt C et al & Dougan G. Targeted Restoration of the Intestinal Microbiota with a Simple, Defined Bacteriotherapy Resolves Relapsing Clostridium difficile Disease in Mice. PLoS Pathog. 2012 8:e1002995. PMID: 23133377
Experimental proof that a rational microbiota can be used to treat infections. Patent from this underpinned the formation of Microbiotica
Everitt AR, Clare S, Pertel T, John SP, Wash RS, Smith SE, Chin CR, et al, Dougan G, Brass AL, Kellam P. IFITM3 restricts the morbidity and mortality associated with influenza. Nature. 2012 484:519-23. PMID:22446628
Nijnik A, Clare S, Hale C, Raisen C, McIntyre RE, Yusa K, Everitt AR, Mottram L, Podrini C, Lucas M et al & Dougan G. The critical role of histone H2A-deubiquitinase Mysm1 in hematopoiesis and lymphocyte differentiation. Blood. 2011, 119:1370-9. PMID: 22184403
Mutreja A, Kim DW, Thomson NR, Connor TR, Lee JH, Kariuki S, Croucher NJ, Choi SY, Harris SR et al &Dougan G. Evidence for several waves of global transmission in the seventh cholera pandemic. Nature. 2011 477:462-5. PMID: 21866102
Definition of the global transmission routes of cholera
Pickard D, Toribio AL, Petty NK, van Tonder A, Yu L, Goulding D, Barrell B, Rance R, Harris D, Wetter M, Wain J et al & Dougan G. A conserved acetyl esterase domain targets diverse bacteriophages to the Vi capsular receptor of Salmonella enterica serovar Typhi. J Bacteriol. 2010 192, 5746-54. PMID: 20817773
Hall LJ, Clare S, Dougan G. NK cells influence both innate and adaptive immune responses after mucosal immunisation with antigen and mucosal adjuvant. J Immunol. 2010 15;184:4327-37. PMID:20220095
Role of mucosal immunity and adjuvants
Langridge GC, Phan M-D, Turner D, Perkins T, Parts L, Haase J, Charles I, Maskell DM, Peters S, Dougan G, Wain J, Parkhill J Turner KA 2009 Simultaneous analysis of one million mutants of Salmonella Typhi. Genome Research, 19:2308-16. PMID:19826075
Kingsley RA, Msefula CL, Thomson NR, Kariuki S, Holt KE, Gordon MA, Harris D, Clarke L, Whitehead S, Sangal V, Marsh K, Achtman M, Molyneux M, Cormican M, Parkhill J, MacLennan CA, Heyderman RS Dougan G 2009 Epidemic multiple drug resistant Salmonella Typhimurium causing invasive disease in Sub-Saharan Africa have a distinct genotype. Genome Research, 19:2279-87. PMID: 19901036
Lawley TD, Croucher NJ, Yu L, Clare S, Sebaihia M, Goulding D, Pickard DJ, Parkhill J, Choudhary J, Dougan G. 2009 Proteomic and genomic characterisation of highly infectious spores from Clostridium difficile. J Bact 191:5377-86. PMID: 19542279
Li P, Burke S, Wang J, Chen X, Ortiz M, Lee SC, Lu D, Campos L, Goulding D, Ng BL, Dougan G, Huntly B, Gottgens B, Jenkins NA, Copeland NG, Colucci F, Liu P. Reprogramming of T cells to natural killer cells upon Bc11b deletion. Science. 2010 2;329:85-9. PMID:20538915
Good EM images of cells contributing to immunity